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Microscopy & lab practice

Using pH paper and reading colour scales

1 min read

In short

pH paper estimates how acidic or alkaline a suitable aqueous sample is through colour change. Select the range, use a clean subsample and compare at the specified time with that product's scale.

Read pH paper: Choose the right range; Use a clean subsample; Follow timing and scale.
Read pH paper: Choose the right range; Use a clean subsample; Follow timing and scale.
Contents
  1. What pH describes
  2. Broad or narrow range?
  3. Avoid common mistakes
  4. Limitations
  5. Questions

What pH describes

For typical aqueous examples around room temperature, below 7 is acidic, around 7 neutral and above 7 alkaline. The scale is logarithmic. It is not a universal hazard scale: similar pH values can accompany very different risks.

Broad or narrow range?

Universal paper, for example 0–14, provides an overview. A narrower range can distinguish smaller differences where appropriate. Read resolution from the supplied scale. Accurate quantitative work may require a suitable calibrated meter.

Avoid common mistakes

  1. Check instructions, expiry and storage.
  2. Where the method allows, transfer a small aliquot into a clean vessel.
  3. Wet the indicator as directed without touching it.
  4. Observe immersion and reading times.
  5. Compare in suitable light and record result and method.

Limitations

Strongly coloured or cloudy samples complicate comparison. Low buffering and unsuitable composition can affect results. Not every paper suits non-aqueous liquids. An uncertain colour must not be converted into unjustifiably precise decimals.

Read vessels and pipettes. Browse laboratory supplies.

Questions

Can I dip into the stock bottle?
An aliquot often prevents contamination. Follow the method, especially for heterogeneous samples.
Can strips be reused?
No; used indicator areas cannot provide reliable new measurements.
Is laboratory paper suitable for medical self-testing?
Only a product specifically intended for that purpose should be used; general laboratory paper is not equivalent.

Read next

Sources & further reading

General guidance for study and teaching. For your actual work, your safety briefing, hygiene plan, lab instructions and the product instructions apply. Legal information refers to Germany.